Thursday 19 March 2015
Tuesday 17 March 2015
Fem Biotika
A Division for Biotechnology
Commercially Important Medicinal & Ornamental Plant Tissue culture:
Fem Analytika micro-propagation department are engaged in tissue culture of banana, Aloe vera, Brahmi, Mandukparni, Shankhpuspi, Stevia and so much medicinal & Ornamental plants. Fem Analytika specializes in micro propagation of flowering, medicinal, temperate green foliages and horticultural products. for buy the plant, contact to Mr. Sachin @ 9899729784 or Mail to us - femanalytika@gmail.com
Job Internship cum Project Training: This program is specially designed for students who want to join Fem Analytika Team or any other research driven industry after completion of their degree program. This is a dual program in which students work with company for 4-6 months and parallel they complete their project work.
Monday 16 March 2015
ADULTERATION IN SPICE (Spice Adulteration)
- Spread approx 10 gram of Mustard black or Fine mustard sample on a white paper.
- Then separate out argemone seed as seen in picture from whole sample and verified with glass lens by care full observation as described in morphological characteristics of Argemone seed.
- Morphological characteristic of Argemone Maxicana - Argemone seeds have roufh groves on upper surface and color is brown to blackish.
Health Effect of Argemone seeds:
The seeds resemble the seeds of Brassica nigra (mustard). As a result, mustard can
be adulterated by argemone seeds, rendering it poisonous. Argemone seeds contains toxic
chemicals, namely Sanguinarine and Dihydrosanguinarine. Consumption of adulterated
mustard seed (Brassica nigra) with argemone seed (Argemone mexicana) even for a short
duration leads to a clinical condition referred as.
- Epidemic dropsy.
Reason to be Adulterated:
Argemone seed is not deliberately added that should be come from agriculture land added but due to high labor charge and illiteracy that should not removed out by manufacture.
3.2. Detection of Lead Chromate as adulterant in Turmeric Whole:
Argemone seed is not deliberately added that should be come from agriculture land added but due to high labor charge and illiteracy that should not removed out by manufacture.
3.2. Detection of Lead Chromate as adulterant in Turmeric Whole:
Saturday 14 March 2015
Bacopa Monnieri (Brahmi) Plant Tissue culture
"Plant Tissue culture
Of
Bacopa monnieri"
(Brahmi)
Steps-1- Treatment & Washing of Explants
- Select a healthy plant from field.
- And collect the shoot parts.
- Wash the shoots thoroughly with running tap water.
- Again wash with few drops of tween-20, thoroughly for removal of fungal or bacterial spores or 1% extran treatment for 10 to 15 min.
- Washed with DDW for 3 to 4 times.
- If treated with 1 % extran then followed by 1% Bavistin treatment for 20 to 30 minutes and again washed with DDW 5 times.
- Transfer the Explant in Pretreated Laminar flow compartment.
Steps-2- Laminar Air flow Treatment
· 1st UV treatment of LAF- Vacant LAF properly rinsed by alcohol and kept 40- 60 min for U.V. Treatment.
· 2nd U.V. Treatment- After 1st U.V. treatment all the required equipment like scalpels, force, sissor, SDDW, petridish and media was surface rinse by alkohal and kept in to LAF for min.
Step-3- surface sterilization & Trimming of Ex-plant under LAF
- Surface sterilized with 0.1% HgCl2 for 5 min followed by 4-5 times rinsing with double distilled water in Laminar air flow.
- Ex-plant was trimmed with the help of scalpel and forceps to remove the exposed part of the explants.
Step-4- Inoculation
· Transfer the material over the flame with the help of forceps in MS media test tube or bottle.
· Cover it with cap.
· Seal it with tape and label the culture vessels with the name of the explants and dote of media preparation and date of inoculation.
Step-5- Transfer in culture Room and culture Room condition
· Material thus inoculated is kept in the culture room (culture room at 25 ± 2 ͦC under 16 hrs light(1000 lux of light intensity and 8 hrs dark condition.
Step -6- sub culturing
· After establishment of culture in the culture tubes they are aseptically transferred or inoculated in wide mouth bottles for further development.
Step-7- Hardening
· After proper development of plantlets, they are transferred to Mist. Chamber/ green house/Shade house for hardening or acclimatization.
====Read More===>>>>
====Read More===>>>>
Subscribe to:
Posts (Atom)